Plasmid Sequence Dataset of Multidrug-Resistant Enterobacterales Isolated from Hospital Effiuents and Wastewater Treatment Plant

Abstract

We present plasmid sequences of 21 multidrug resistant iso- lates of Enterobacterales belonging to Escherichia coli (n=10), Klebsiella pneumoniae (n=9), Klebsiella oxytoca (n=1), and Cit- robacter freundii (n=1). The isolates originated from effiuent collected from hospital sewer pipes and from a wastewa- ter treatment plant (WWTP) in a southwestern Hungarian city. Isolation was carried out using eosin methylene blue agar supplemented with ceftriaxone and the isolates were identified with MALDI-TOF MS. Screening for multidrug re- sistance was conducted by determining susceptibility to four chemical classes namely, beta-lactams, aminoglycoside, flu- oroquinolone, and sulfonamide. Plasmid DNA was isolated by the alkaline lysis method using the Monarch plasmid DNA miniprep kit from freshly grown pure colonies. Molecular typing and Illumina sequencing of plasmid DNA of mul- tiresistant strains were performed. After the assembly of contigs, genes localized on plasmid sequences were deter- mined and functionally annotated. These reconstructed plas- mid sequences supplemented with gene functional anno- tations were deposited in the Mendeley data. Using these datasets different plasmid incompatibility groups were iden- tified. These conjugative plasmids appear to play a key role in the transmission of multiple resistance genes in enteric bac- teria via wastewater. The presented data may provide useful insight on the correlations between environmental antibiotic contamination and the development of bacterial resistance, which poses a serious public health threat.