Browsing by Author "Zhang, Yingmiao"

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    Distinct Patterns of Host Adherence by Neisseria gonorrhoeae Isolated from Experimental Gonorrhea
    (Canadian Journal of Infectious Diseases and Medical Microbiology, 2021-05-15) Yingxia, He; Zhang, Song; Zhang, Yingmiao; Bicong, Wu; Xue, Ying; Chenglin, Ye; Qiao, Li; Adhiambo, Njiri Olivia; Tembo, John Mambwe; Hongxiang, Chen; Huahua, Cai; Tie, Chen
    Neisseria gonorrhoeae (N. gonorrhoeae, gonococci, or GC), the etiologic agent of gonorrhea, is a human-obligate bacterial pathogen. -e GC surface contains pili that mediate the adherence to host cells. Studies have shown that GC pili, coded by pilin genes, undergo remarkable changes during human experimental gonorrhea, possibly generated by DNA phase variation during infection. -e question that arises is whether the changes in pilins can alter the adherence capacity of N. gonorrhoeae to host cells. In this study, six variants initially isolated from male volunteers infected with one single clone of GC were examined for their adherence patterns with human Chang conjunctiva cells. In this study, we showed that the variants showed distinct adherence patterns to this cell line under light microscopy and scanning electron microscopy. Moreover, two reisolates showed higher adherence capacities than that of the input strain. -e results provide an additional example as to how the pilus variation may play a role in the pathogenesis of N. gonorrhoeae.
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    Salmonella enterica Serovar Typhimurium Interacts with CD209 Receptors To Promote Host Dissemination and Infection
    (American Society for Microbiology, 2019-07-23) Ye, Chenglin; Li, Qiao; Li, Xinyi; Park, Chae Gyu; He, Yingxia; Zhang, Yingmiao; Wu, Bicong; Xue, Ying; Yang, Kun; Lv, Yin; Ying, Xiao-Ling; Ding, Hong-Hui; Cai, Huahua; Alkraiem, Ayman Ahmad; Njiri, Olivia; Tembo, John; Huang, Hong-Ping; Li, An-Yi; Gong, Jianping; Qin, Jichao; Cheng, Bing; We, Xiang; Sun, Ziyong; Zhang, Shu-Sheng; Zhang, Pei; Zheng, Guo-Xing; Li, Wei; Kan, Biao; Yan, Meiying; Xiding, Xiamu; Huo, Xixiang; Huo, Xixiang; Peng, Hua; Fu, Yangxin; Klena, John D.; Skurnik, Mikael; Jiang, Ling-Yu; Chen, Tie
    Salmonella enterica serovar Typhimurium, a Gram-negative bacterium, can cause infectious diseases ranging from gastroenteritis to systemic dissemination and infection. However, the molecular mechanisms underlying this bacterial dissemination have yet to be elucidated. A study indicated that using the lipopolysaccharide (LPS) core as a ligand, S. Typhimurium was able to bind human dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (hCD209a), an HIV receptor that promotes viral dissemination by hijacking antigen-presenting cells (APCs). In this study, we showed that S. Typhimurium interacted with CD209s, leading to the invasion of APCs and potentially the dissemination to regional lymph nodes, spleen, and liver in mice. Shielding of the exposed LPS core through the expression of O-antigen reduces dissemination and infection. Thus, we propose that similar to HIV, S. Typhimurium may also utilize APCs via interactions with CD209s as