Browsing by Author "Musalia, L. M."

Now showing 1 - 3 of 3

Carcass Fatty Acid Composition and Sensory Properties of Nile Tilapia (Oreochromis Niloticus) Fed on Oilseed Meals With Crude Papain Enzyme
(East African Journal of Science, Technology and Innovation, 2022) Kirimi, J. G.; Musalia, L. M.; Munguti, J. M.; Magana, A.
In this study, proximate body composition, fillets fatty acid and sensory properties of Nile tilapia fed on oilseed meals with crude papain enzyme were determined. A control diet (D1) of 300g/kg crude protein (CP) and (2900Kcal/kg) was formulated using fishmeal (Rastrionaebola argentea) (FM) and test diets by replacing 10% CP of FM by soybean meal (SBM) (D2), canola meal (CM) (D3) and sunflower meal (SFM) (D4). One hundred and one days feeding trial was conducted in a 4x2 factorial design on 4 diets (D1, D2, D3 and D4) with (0.06%) and (0%) enzyme using 720 Nile tilapia fingerlings (7±3g). Fish were fed twice daily at 5% of their biomass at 10am and 4pm in two equal meals. At the end of feeding trial, fish were starved for 24 hrs and weighed. A sample of ten fish representing the average weight of each replicated group was used. The fillets fatty acid profiles were determined by MPA FT-NIR spectrometer. Organoleptic tests of both fresh and steamed fish were carried out by eight semi trained panellists using 5-point hedonic scale. There was increase (p<0.05) in carcass proximate composition in all the treatments. Fillets fatty acid levels were influenced by the crude papain enzyme (p<0.05). Palmitic acid (C16:0), linoleic acid (C18:2n-6) and oleic acid (C18:1n-9) were the most abundant fatty acids in the fillets. Fish fed on fishmeal based diet recorded higher percentage (22.56) of polyunsaturated fatty acids (PUFA). There was significant difference among the treatments (p<0.05) for general appearance. Fish fed on crude papain enzyme treated diets were less preferred. It is recommended that more research be done on the effect of papain enzyme on the carcass fatty acid and sensory properties of farmed fish.
Effect of Substituting Fishmeal with Oilseed Meals on Diets Fatty Acid and Proximate Composition for Nile tilapia (Oreochromis niloticus)
(Journal of Agriculture and Veterinary Science, 2021) Kirimi, J. G.; Musalia, L. M.; Munguti, J. M.; Magana, A.
The study aimed to evaluate the fatty acid and proximate composition of diets for Nile tilapia containing soybean meal (SBM), canola meal (CM) and sunflower meal (SFM) as replacements of fishmeal (FM). A control diet (D1) of 30% crude protein (CP) was formulated using fishmeal as main protein source. The test diets (D2, D3 and D4) were formulated by replacing 10% CP of FM by SBM, CM and SFM, respectively. The fatty acid profile of ingredients and diets were determined by MPA FT-NIR spectrometer. FM displayed higher CP content (62.60%) followed by SBM (47.38%), CM (34.39%) and SFM (24.81%). SFM had highest crude fibre content (p<0.05) while CM displayed higher figure for ether extracts (p<0.05). Substituting FM with SBM, CM and SFM increased the levels of crude fibre (p<0.05). Diet 4 recorded highest crude fibre (16.03%) content (p<0.05), while CM based diet recorded highest ether extract content (10.75%), (p<0.05). Diet 1 had lowest concentration (21.85mg/100g) of total saturated fatty acid and D2 lowest concentration of polyunsaturated fatty acid (29.90mg/100g). The study revealed that 10% CP substitution of FM with SBM, CM and SFM in Nile tilapia diets is possible without much negative change in diets proximate and fatty acid composition.
Nutrients Digestibility and Growth Performance of Nile Tilapia (Oreochromis Niloticus) Fed on Oilseed Meals with Crude Papain Enzyme
(Wiley, 2022) Kirimi, J. G.; Musalia, L. M.; Magana, A.; Munguti, J. M.
In this study, nutrients digestibility and growth performance of Nile tilapia (Oreochromis niloticus) fed on oilseed meals with crude papain enzyme were determined. A con- trol diet (D1) of 30% crude protein (CP) was formulated using fishmeal (FM), soybean meal (SBM), canola meal (CM) and sunflower meal (SFM). The test diets were formu- lated with replacing 10% CP of FM by SBM (D2), CM (D3) and SFM (D4), respectively. Crude papain enzyme in powder form was incorporated at 0.06%. Digestibility trial was performed using chromium oxide as an inert marker. A feeding trial of 101 days was conducted using a 4 × 2 factorial design with 4 diets (D1, D2, D3 and D4) and 2 papain enzyme inclusion levels at 0.06% and 0% using 720 Nile tilapia fingerlings (7 ± 3 g). The fingerlings were randomly distributed into 8 groups of 3 replicates of 30 fingerlings per net hapa (2 × 1 × 1 m3). Fish were fed twice daily at 5% of their biomass at 10 AM and 4 PM in two equal meals. Apparent digestibility coefficients increased (p < 0.05) on crude papain enzyme supplementation. There was increase in final body weight (47.32 ± 2.10 g) on 0.06% enzyme than without enzyme (0%) (46.17 ± 2.14 g) (p > 0.05). Irrespective of enzyme supplementation, fish fed FM-based diet were larger (56.89 ± 1.37 g) (p < 0.05) than those fed on oilseed meals; SBM (45.59 ± 0.91 g), CM (43.89 ± 2.12 g) and SFM (40.59 ± 1.60 g). Based on the study, 0.06% crude papain enzyme inclusion increased nutrients digestibility and growth per- formance of Nile tilapia. Therefore, crude papain enzyme is recommended as a feed additive in Nile tilapia diets to promote growth. However, more research is recom- mended to determine optimum inclusion levels of crude papain enzyme in Nile tilapia diets.