Browsing by Author "Getenga, Z.M."
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Item Distribution and Diversity of Antibiotic Resistant Bacteria in Selected Agro-Industrial Pollution in Njoro River, Nakuru, Kenya(Chuka University, 2016) Itotia, T.K.; Muia, A.W.; Kiruki, S.; Getenga, Z.M.Many in-stream activities occur in River Njoro. Consequently, many pharmaceuticals used in farms and hospitals and antibiotic-resistant microbes end up in the River through runoff and sewage. Presence of antibiotic resistance exposes humans and animals to contamination during in-stream activities. This study determined bacteria resistant to both medical and veterinary antibiotics in the catchment. Residual antibiotics and physical chemical conditions and indicators of feacal pollution were investigated. The bacteria resistant to five antibiotics studied varied significantly (P<0.05). Turkana site had highest resistors to ampicillin, tetracycline and streptomycin, while Njoro Canning Factory had highest resistors to gentamycin and Chloramphenical. Indicators of faecal pollution were found in all sites including Sigotik with 413.33±15.28 E.coli per 100 ml of water. Physical chemical measurements showed site differences. The Njoro Canning Factory BOD was 6.99±0.20 mg L-1, whereas Sigotik BOD was 1.28±0.13 mg L-1. Presumptive positive Salmonella, V. cholera and V. parahaemolyticus species were found in Turkana and Ngata sites. There is cause for alarm due to the high numbers of antibiotic resistant bacteria in River Njoro. Proper treatment of the River water before use is recommended, or alternative safe water sources for these communities should be found.Item Phthalate Levels in Wastewater Treatment Plants of Lake Victoria Basin(Science and Education Publishing, 2021) Onchiri, R.; Ongulu, R.; Orata, F.; Getenga, Z.M.; Gichumbi, J.M.; Ogora, E.N.Phthalates are examples of EDCs, which have been used as plasticizers for longtime worldwide. However, their occurrence in these environments causes adverse effects such as congenital anomalies, endocrine disruption and chronic toxicity. Wastewater is the source of these phthalates in the environment. The main aim of this study was to determine the levels of dimethyl phthalate (DMP), benzyl butyl phthalate (BBP) and bis (2-ethylhexyl) phthalate (BEHP) in wastewater sampled from wastewater treatment plants (WWTPs) of Nyalenda, Homabay and Kisii, in Kenya during wet and dry seasons. Thermos Scientific Dionex UltiMateTM 3000 HPLC system was used to analyze the residue levels at 0.004 and 0.005 mg/L limits of quantification during wet and dry seasons, respectively. Most sites sampled had detectable levels of DMP, BBP and BEHP. Higher concentrations were detected during wet season compared to dry season. At Nyalenda treatment plant, DMP was detected at 0.99 ± 0.0005 mg/L and 0.79 ± 0.0005 mg/L at the inlet sampling point during the wet and dry seasons, respectively. The concentrations detected were high at the inlet and lowest at the outlet sampling points. The residue levels obtained showed significant differences at 5% confidence limits with Zcalculated for DMP at Nyalenda, Homabay and Kisii WWTPs obtained as 0.2650, 0.2183 and 0.1983, respectively. These Z-score values (Zcalculated) were less than the critical value (Zcritical = 1.96). Similar observations were observed with BBP and BEHP.