Browsing by Author "Fredrick O. Ogolla"

Now showing 1 - 6 of 6

BACTERIOLOGICAL QUALITY STATUS OF BOREHOLES WATER IN THARAKA NITHI COUNTY, KENYA
(Abjournals, 2020) Fredrick O. Ogolla; Kithaka Samson Chabari; Jedidah Karimi Kariuki
Water shortage that is caused by long droughts and higher cost of accessing piped water have forced many households in Kenya to opt for wells and borehole water. Where they are used, boreholes are exposed to contamination by pathogenic microorganisms from nearby toilets, defecation from domesticated animals and surface runoff. Despite of known health concerns of consuming contaminated water, many rural residents still consume borehole water whose portability is not determined and may not meet WHO standards. Thus, this study was conducted in Tharaka Nithi County to assess bacterial quality status of selected boreholes in Maara, Igamba Ngombe and Tharaka Constituencies between April to September 2019. A total of 108 water samples were collected from 36 boreholes. Boreholes in Mukothima within Tharaka area were not collected as the ones accessed were dry at the time of sampling. The samples were analyzed at Chuka University using most probable number methods, Plate count and bacteria species identified using selected biochemical methods. Data (MPN) for total and feacal coliform for different location of study and boreholes were log transformed (log10 (cfu+3) and compared using general linear method in SAS. Significance means were separated using least significant difference post hoc test in SAS version 9.4. Number of bacteria isolated from water samples were also compared using general linear model. Feacal coliform values exceeding WHO recommendation of (0 CFU/ 100 ml was observed in all borehole surveyed. Mean MPN value of 17.5/ 100 ml CFU was observed in Kawangware. Coliform bacteria E. coli, Bacillus spp, Klebsiella spp were isolated in all the water sampled. However, E. coli followed by Bacillus spp were higher compared to the rest of isolates. Occurrence of these bacteria in borehole water put the health of depended consumers at risk of infection.
Bacteriological Water Quality Assessment Of Nkenye Stream In Meru South In Tharaka Nithi County, Kenya
(Research journali, 2020-02) Kithaka Samson Chabari; Erick C. Njagi; Adiel Magana; Fredrick O. Ogolla
Access to portable water remains a major global concern due to increased rate of water pollution contributed for by climate change and human. Destruction of riparian zones has exposed the rivers and streams to toxic and pathogenic pollutants released from untreated organic and inorganic waste. Exposure of river and stream used for drinking water to pollution is detrimental to aquatic plants, animals, and human consumers who depends on such valuable ecosystem. Nkenye stream that originates from Nkenye wetlands in Chuka, Meru South provide water that is used in fish ponds, irrigation and water for domestic use in homestead. Despite of the significance of Nkenye stream, little attention has been accorded to ascertain its water quality given its location. Study was conducted to determine the bacteriological water quality of Nkenye stream based on the feacal and total coliform count. Samples were collected at designated locations using ecological survey method and taken to Chuka University for evaluation. Coliform analysis of water samples was done by most probable number method using Macconkey purple broth at botany laboratory, Chuka University. The results of coliform counts obtained were subjected to the analysis of variance using General linear model (GLM) on Statistical analysis system (SAS) version 9.4. The significance coliform means were separated by Least significance difference (LSD) [alpha = 0.05]. Feacal coliform count of Nkenye stream did not conform to WHO standards of 0 cfu/100 ml as the mean of 10 cfu/100 ml was observed for the entire stream. The occurrences of these bacteria in water indicate water deterioration and the presence of many water-borne pathogens that needs immediate attention. The study recommends that local authorities particularly Tharaka Nithi county government should provide waste management disposal systems and policies that prohibit direct discharge of untreated effluents to Nkenye stream.
Cultural, Morphological and Biochemical Identification of Xanthomonas Spp the Causative Agent of Bacteria Leaf Spot in Tomatoes in Wanguru, Mwea, Kirinyaga County, Kenya
(rsisinternational, 2019-04) Fredrick O. Ogolla; DaisyB. Neema; ; ;
Bacteria diseases are major constraints in commercial agriculture. Bacteria diseases have continuously caused huge loss to both conventional and organic farming systems globally. Bacteria leaf spot is a tomato disease of concern whose management remains a challenge across the globe. The pathogen Xanthomonas campestris pv vesicatoria the causative agent of leaf spot in tomato has been isolated and positively identified in many countries to enable control. In Kenya, tomato farmers have experienced losses associated with bacterial diseases despite using available control strategies. Application of control mechanisms by majority of farmers heavily rely on symptoms on plants other than laboratory facts on pathogen identification. As such wrong chemicals have been applied which have led to development of resistance due to pathogen evolution. Scientific identification of pathogen is necessary to enable application of accurate and effective management strategy. Nonetheless, this has scarcely been done for tomato bacteria diseases in Kenya. The objective of this study was to isolate and identify bacteria leaf spot pathogen of tomato from Wanguru area in Mwea, Kirinyaga county in Kenya using nutrient agar, differential staining and selected biochemical methods. A total of ten tomato heavily infected leaves were selected randomly from different farms. The colonies were generally yellow on the surface of nutrient agar. Results of differential staining showed gram negative rods while biochemical tests slightly varied. Based on totality of cultural, morphological and biochemical tests results, we concluded that Xanthomonas campestris pv vesicatoria is responsible for leaf spot tomato disease in Wanguru. However, we recommend the inclusion of molecular tool for proper identification.
Determination Of Effect Of Land Use On Distribution And Abundance Of Ground Dwelling Macro Invertebrates In Kirimiri Forest In Embu County, Kenya
(International Journal of Advanced Research and Publications, 2020-03) Clifton Omondi; Fredrick O. Ogolla; Christopher Odhiambo
Ground dwelling macro invertebrates are essential for soil functions and other significant ecological process such as nutrient cycling. The distribution and ecological role of crawling macro invertebrates may be influenced by anthropogenic factors. Human factors such as deforestation and agricultural activities that destroy the habitat pose great threat for the survival of macro invertebrates. Most of the natural ecosystems including forests in Kenya have been encroached, segmented and reduced in size by the rapidly growing population. However, studies on the impact of such destructive activities on the abundance and distribution of ground dwelling macro invertebrates are limited. Thus, there exist information gap on macro invertebrate composition and their distribution in different ecosystem and habitat segments in Kenya. Such studies are necessary in generating knowledge and creating wholesome understanding to facilitate policy making, habitat management and conservation of crawling macro invertebrates. Based on the above highlights, this study was conducted to determine the effect of land use on the distribution and abundance of ground dwelling macro invertebrate in Kirimiri forest in Embu County, Kenya between January and April 2016. The Napier grass plantation, Tea plantation and indigenous intact forest were evaluated for their macro invertebrates. In every habitat studied, crawling macro invertebrates were caught using the pit fall traps set in 50 m by 50 m grid subdivided into six rows at equidistance gap of 8 m. The pit holes comprised of 60 (250 ml capacity) clear plastic containers filled with 30 ml mixture of ethanol and liquid soap. Macro invertebrates were identified using their morphometric features and then stored in 70 % Ethanol for further laboratory identification at the National museums of Kenya headquarter in Nairobi, Kenya. The data collected was analyzed using Scientific Analysis System (SAS) version 9.4 and significance means separated using Least Significance Difference (SLD). The indigenous intact forest recorded the highest mean of macro invertebrates with family of Polydesmidae being the most abundant (mean=17.33). Tea plantation had the second largest mean (4.59) of macro invertebrates, and the family Gryllidae was the most abundant group with mean of 12.667. Napier grass plantation had a mean of 3.94 and the family Platydesmidae was the most abundant group (mean=12.833). The disparity in abundance and distribution of terrestrial macro invertebrate observed in this study may have resulted from micro climate and microenvironment shift influenced by human activity along and within the forest.
Prevalence of banana Xanthomonas wilt in Nithi, Tharaka-Nithi County in Kenya
(Journal BiNET, 2021-05-30) Mogaka M. Onyambu; Benson O. Onyango; Moses M. Muraya; Peterson M. Ong’au; Fredrick O. Ogolla; ; ;
Banana (Musa spp.) is a nutrient rich crop grown in small and large scale across the globe. It is rich in carbohydrates, vitamins and minerals thus, it may serve as the source of nutrition in low resourced regions such as Tharaka Nithi County, Kenya. However, banana production in many areas is faced with biotic constraints such as banana Xanthomonas wilt (BXW) disease caused by Xanthomonas campestris pv. musacearum. The pathogen attacks photosynthetic leaves and young fruits, reducing palatability and marketability. There is currently scanty information on prevalence of BXW in Nithi region of Tharaka Nithi County. Additionally, it remains unclear whether farmers in the region are knowledgeable on occurrence of BXW disease, symptoms and management practices. The prevalence of BXW was assessed through survey method in five villages (Kiang’ondu, Marima, Mitheru, Kibumbu and Giampampo) in Nithi. Percentage BXW prevalence in the studied villages was not significantly different (p>0.05). However, slightly higher BXW prevalence value of 21.14% was recorded at Giampampo village and was lower at Mitheru 11.24%. There was significant relationship (X2 (8, N = 46) = 19.93, p= 0.0034) between banana variety grown and occurrence of banana diseases. There was significant relationship (X2 (8, N = 46) = 31.165, p = 0.0053) between banana variety grown and susceptibility to diseases. The relationship between years of growing banana in the same farm and occurrence of banana diseases was significant (X2(6,N= 46) = 8.761,p= <0.0001. The relationship between occurrence of banana diseases and season of the year was significant (X2(8,N= 46) = 32.4591, p= <0.0001). The relationship between occurrence of banana diseases and management option in Nithi was significant (X2(4,N= 46) = 6.9758,p= 0.0025). Majority of the farmers (92%) were unaware of existence of BXW in the region. Farmers expressed lack of knowledge on managing BXW diseases in their banana farms with the majority not being able to identify common diseases of banana. Based on these observations, there is need to educate farmers on best banana farming practices, disease identification and management.
Screening of Antimicrobial Activity of Poly Herbal Extracts against Bacterial Pathogens Causing Gastroenteritis in Tharaka Nithi County, Kenya
(South Asian Journal of Research in Microbiology, 2023-04-27) Mbithe P. Kiteme; Benson O. Onyango; Eric C. Njagi; Fredrick O. Ogolla
Herbals have been used for decades to treat gastrointestinal infections across the world, including in Tharaka-Nithi County, Kenya. Crude extracts from herbs have long been prescribed by traditional healers for treatment of typhoid, cholera, and shigellosis. However, the effectiveness of extracts obtained using different extraction solvents such as methanol, acetone, and hexane have not fully been evaluated. This study aimed at evaluating the effect of solvents (Ethanol, hexane, and methanol) on the yield of crude extract from plants (Erythrinna abyssinica, Aspilia pluriseta, Vangueria infausta, Ficus sycomorus, and Carissa edulis), assessing the effect of the solvents on extracted active metabolites, and determining the effect of these solvents on extract’s bioactivity against Vibrio cholerae, Shigella flexneri, and Salmonella typhimuriumi and, Escherichia coli that served as a model organism for screening plant extracts against Gram-negative bacteria. Crude extracts were obtained by soaking dried ground plant parts in individual solvents, which were then concentrated by a rotary evaporator. The phytochemical screening to detect plant metabolites was done qualitatively. Bioassays to analyze the efficacy of the plant crude extracts against the microbes were carried out in 4×3×3×5 factorial experiment laid out in a completely randomized design. The determination of bioactivity of herbal extracts was carried out using minimal inhibition concentration and minimum bactericidal concentration methods. Data obtained on the bioactivity assay (Count of the bacteria colony forming units) was analyzed using the Kruskal-Wallis test at α = 0.05, and medians were compared by the Wilcoxon rank sum test in Scientific Analysis System version 9.4. Methanol solvent produced higher quantities of crude extracts for all the herbal samples used with, Vangueria infausta producing the highest extract (5.06g). Most phytochemicals were present in Methanolic extracts compared to hexane and acetone extracts. There was a significant (p < 0.05) difference in the bioactivity of different herbal plants against bacterial pathogens at different concentrations of crude extract. The efficacy of plant extract increased progressively from 100ppm to 1000ppm concentration. Methanol is recommended for use in the extraction of medicinal plant extracts as it leads to improved potency as compared to hexane and acetone.