dc.contributor.advisor | | English |
dc.contributor.advisor | | |
dc.contributor.advisor | | English |
dc.contributor.author | Martin Kagiki, Njogu | |
dc.contributor.author | Fan, Yang | |
dc.contributor.author | Ji, Li | |
dc.contributor.author | Xueyan, Wang | |
dc.contributor.author | Joshua Otieno, Ogweno | |
dc.contributor.author | Jinfeng, Chen | |
dc.date.accessioned | 2023-09-25T08:25:57Z | |
dc.date.available | 2023-09-25T08:25:57Z | |
dc.date.issued | 2020 | |
dc.identifier.uri | http://repository.chuka.ac.ke/handle/chuka/15720 | |
dc.description.abstract | Determinate and indeterminate growth habits of cucumber can affect plant architecture and crop yield. The TER MINAL FLOWER 1 (TFL1) controls determinate/indeterminate growth in Arabidopsis. In this study, a novel mutation in
cucumber TFL1 homolog (CsCEN) has shown to regulate determinate growth and product of terminal fowers in cucumber
(Cucumis sativus L.), which is similar to the function of CsTFL1 as previously reported. Genetic analysis in two determinate
genotypes (D226 and D082) and indeterminate genotype (CCMC) revealed that a single recessive gene is responsible for this
determinate growth trait. With the combination of BSA-seq and whole-genome resequencing, the locus of determinate-novel
(det-novel) trait was mapped to a 44.5 kb genomic region in chromosome 6. Sequence alignment identifed one non-synony mous SNP mutation (A to T) in the third exon of CsCEN, resulting in an amino acid substitution (Thr to Pro), suggesting that
determinate growth might be controlled by a novel gene CsCEN (Csa6G152360) which difered from the reported CsTFL1
gene. The CsCEN expression level in shoot apexes and axillary buds was signifcantly lower in D226 compared to CCMC,
suggesting its essential role in sustaining indeterminate growth habit. Identifcation and characterization of the CsCEN in
the present study provide a new insight into plant architecture modifcation and development of cucumber cultivars suited
to mechanized production system. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Springer-Verlag GmbH Germany, part of Springer Nature | en_US |
dc.relation.ispartofseries | Theoretical and Applied Genetics; | |
dc.subject | Key message BSA-seq combined with whole-genome resequencing map-based cloning delimited the cucumber det novel locus into a 44.5 kb region in chromosome 6 harboring a putative candidate gene encoding a phosphatidyleth anolamine-binding protein (CsCEN). | en_US |
dc.title | A novel mutation in TFL1 homolog sustaining determinate growth in cucumber (Cucumis sativus L.) | en_US |
dc.type | Article | en_US |