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dc.contributor.advisorEnglish
dc.contributor.advisor
dc.contributor.advisorEnglish
dc.contributor.authorMartin Kagiki, Njogu
dc.contributor.authorFan, Yang
dc.contributor.authorJi, Li
dc.contributor.authorXueyan, Wang
dc.contributor.authorJoshua Otieno, Ogweno
dc.contributor.authorJinfeng, Chen
dc.date.accessioned2023-09-25T08:25:57Z
dc.date.available2023-09-25T08:25:57Z
dc.date.issued2020
dc.identifier.urihttp://repository.chuka.ac.ke/handle/chuka/15720
dc.description.abstractDeterminate and indeterminate growth habits of cucumber can affect plant architecture and crop yield. The TER MINAL FLOWER 1 (TFL1) controls determinate/indeterminate growth in Arabidopsis. In this study, a novel mutation in cucumber TFL1 homolog (CsCEN) has shown to regulate determinate growth and product of terminal fowers in cucumber (Cucumis sativus L.), which is similar to the function of CsTFL1 as previously reported. Genetic analysis in two determinate genotypes (D226 and D082) and indeterminate genotype (CCMC) revealed that a single recessive gene is responsible for this determinate growth trait. With the combination of BSA-seq and whole-genome resequencing, the locus of determinate-novel (det-novel) trait was mapped to a 44.5 kb genomic region in chromosome 6. Sequence alignment identifed one non-synony mous SNP mutation (A to T) in the third exon of CsCEN, resulting in an amino acid substitution (Thr to Pro), suggesting that determinate growth might be controlled by a novel gene CsCEN (Csa6G152360) which difered from the reported CsTFL1 gene. The CsCEN expression level in shoot apexes and axillary buds was signifcantly lower in D226 compared to CCMC, suggesting its essential role in sustaining indeterminate growth habit. Identifcation and characterization of the CsCEN in the present study provide a new insight into plant architecture modifcation and development of cucumber cultivars suited to mechanized production system.en_US
dc.language.isoenen_US
dc.publisherSpringer-Verlag GmbH Germany, part of Springer Natureen_US
dc.relation.ispartofseriesTheoretical and Applied Genetics;
dc.subjectKey message BSA-seq combined with whole-genome resequencing map-based cloning delimited the cucumber det novel locus into a 44.5 kb region in chromosome 6 harboring a putative candidate gene encoding a phosphatidyleth anolamine-binding protein (CsCEN).en_US
dc.titleA novel mutation in TFL1 homolog sustaining determinate growth in cucumber (Cucumis sativus L.)en_US
dc.typeArticleen_US


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