Shikuku B. O.Kiruki S.Kuria E.2025-04-092025-04-092023Shikuku B. O., Kiruki S. And Kuria E. (2023). Characterization of Antibiotic Producing Actinomyces from River Tana and Lake Elementaita. In: Isutsa, D. K. (Ed.). Proceedings of the Chuka University 9th Annual International Research Conference held in Chuka University, Chuka, Kenya from 24th to 25th November, 2022. 360-369pp.https://repository.chuka.ac.ke/handle/123456789/17624bshikuku@chuka.ac.ke; skiruki@chuka.ac.ke; ekuria@chuka.ac.keThe aquatic environment has many Actinomyces such as Streptomyces species, that produces anti-tumors, enzymes, antibiotics, antivirals, and antifungals. Actinomyces species have produced antibiotics such as chloramphenicol, streptomycin, gentamycin, among others. The increased prevalence of bacterial infections has been a major challenge to the human population with devastating high morbidity and mortality rates. This situation has been worsened by increasing antibiotic resistant strains of pathogenic bacteria, reduced effectiveness of antibiotics in the market, and the emergence of new bacterial infections. This study aimed at isolation and molecular identification of antibacterial Actinomyces species. The experiments for this study was laid out in Complete Randomized Design and replicated three times to determine the difference between the inhibition zones (mm) of isolates against the tests organisms. A total of six antibiotic producing Actinomyces species were isolated from river Tana and lake Elementaita and identified through morphological and molecular characterizations. There was significant (p<0.05) difference on antibacterial activity of Actinomyces isolates against Escherichia coli, Salmonella typhi and Staphylococcus aureus. The findings of this study can help in developing new or alternative antibiotics that can be used for treatment of pathogenic and resistant bacteria.enSix Actinomycesantibiotic resistanceantibacterial activity.Article